TECHNICAL DESCRIPTION

The present invention relates to a novel yeast bilirubin oxidase, to the method for preparing this BOD and also to its use, in particular for assaying bilirubin and for its use in enzymatic biofuel cells to reduce oxygen.
This new yeast enzyme would be also helpful for understanding molecular mechanisms involved in oxygen reduction by multicopper oxidase.

INDUSTRIAL APPLICATIONS

Biofuel cells, oxygen biosensors, bilirubin biosensors, commercial enzyme for fundamental and academic research, wood pulp treatment and dyes decolourization.

STATE OF DEVELOPMENT

We already demonstrated the concept and the enzyme is available for testing.

ADVANTAGES

We have shown that this new fungal Bilirubin oxidase presents 5 main advantages compared to the previous commercial enzyme from Myrothecium verrucaria (Sigma-Aldrich).

Highest stability and activity in physiological conditions (0.14 M NaCl, pH 7);

Higher stability and activity at 60°C;

Large production of enzyme and easiness of production and purification;

Better catalytic activity with substrates such as ABTS and Bilirubin;

Highest electroreduction current of O₂ reached on various electrodes surfaces either in Direct electron transfer (DET) or in Mediated electron transfer (MET) in physiological conditions.