Method for screening anticancer substances, set or kit for implementing said method
Référence
86825-01
Mots-clés
Screening, Cancer, Cell culture, Living cells, E-cadherin, Métastasis
Statut des brevets
French patent application BF0551616 filed on juin 14th, 2005 and entitled « Technique de criblage fonctionnel de molécules bioactives anti-métastasiques sur cellules épithéliales invasives »
Inventeurs
Pierre ROUX
Marion DE TOLEDO
Statut commercial
Licensed
Laboratoire
Centre de Recherche de Biochimie Macromoléculaire (CRBM), a CNRS laboratory (UMR5237), in Montpellier, France.
Description
TECHNICAL DESCRIPTION
The inventors have developed a new method for screening anticancer substances. This protocol is based on the colon cancer model cells SW620 and the observation that neoformation of E-cadherin type junctions is in correlation with metastases reduction.
This protocol includes the following steps:
- obtaining a culture of tumoral cells not expressing E-cadherin on their cell membrane
- contacting the cells obtained at step a) with a candidate substance or a combination of candidate substances
- detecting, in the culture of cells obtained at the end of step b), the presence of E-cadherin at the cell surface
- positively selecting the candidate substance or combination of candidate substances when E-cadherin has been detected at step c).
INDUSTRIAL APPLICATIONS
An important advantage of this new method is to reduce the cost and the delay in new therapeutic molecules screening.
Drug screening for developing anti-metastatic therapy protocols.
For further information, please contact us (Ref 86825-01)
Besoin de plus d'informations ?
Nous contacterTechnologies Liées
-
24.02.2015
High Throughput Screening of LINGO Ligands: A New Drug Discovery Tool for the Treatment of Neurodegenerative Diseases
Outils de recherche et criblage 03247-01
-
20.02.2014
New cellular and animal toxic model of Parkinson’s disease
Outils de recherche et criblage 05155-01
-
11.02.2014
The frequency of cellular nodules as a new marker for metastase in colorectal cancer
Outils de recherche et criblage 85349-01